Preparation of 2-DE Samples

Kidney cortex with normal appearance from patients under nephrectomy due to renal tumor

Partially purified glomeruli

A standard sieving method

Highly purified glomeruli

Picking-up under a phase-contrast microscopy

2-DE sample

Lysis solution: 9.8 M urea, 2 % NP-40, 2 % Pharmalyte, pH 3-10, 100 mM DTT, 0.5 µg/ml E-64, 0.5 mM PMSF, 40 µg/ml TLCK, 1 µg/ml aprotinin, 10 µg/ml cymostatin, 0.5 mM EDTA

Solubilized with lysis solution